Fk2 antibody

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August undefined, 2024

WebGeneral description. Mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes K 29 -, K 48 -, and K 63 -linked polyubiquitinylated and monoubiquitinylated proteins. Recognizes K 29 -, K 48 -, and K 63 -linked polyubiquitinylated and monoubiquitinylated ... WebJan 7, 2024 · The hybridoma secreting the antibody FK2 was generated by fusion of splenocytes from Balb/c mice which had received repeated immunization with a crude preparation of polyubiquitinylated-lysozyme. The antibody (clone FK2) has been extensively characterized by one-dimensional Western blotting. The immunoglobulin …

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WebApr 14, 2024 · Ubiquitinated proteins, detected by the monoclonal antibody FK2 (ub-FK2), and RNF8-dependent ubiquitinated H2A (ub-H2A, detected by monoclonal antibody E6C5) are localized on the sex body area (Lu et al., 2010). K48- and K63-linked poly-ubiquitins are enriched in the XY body (Lu et al., 2010). The role of ubiquitination around the XY body ... Web(B and C) Mono- and poly-ubiquitinated proteins detection using the FK2 specific monoclonal antibody (B) Quantification of ubiquitinated protein detection after normalization to Actin (C). (D) Control and treated RHEs were stained with haematoxylin–eosin (n = 2, 1 independent bank). Bar = 10 μm. easy get credit cards bad credit

Fk2 Monoclonal Antibody Biomol GmbH Bioz

Web(C) Ubiquitin IP with the FK2 antibody from the cells expressing the indicated proteins, followed by IB with FK2 and the pan-ubiquitin antibody #3933. (D) IP using the HA antibody from the cells co-expressing HA-ubiquitin with the indicated proteins, followed by IB with the HA and pan-ubiquitin antibodies. WebFeb 11, 2024 · Anti-Multi Ubiquitin mAb(Monoclonal, FK2 from MBL.Clone FK2 has been reported to recognize K29-, K48-, K63-linked poly ubiquitinylated and mono … WebJan 7, 2024 · product name : Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2) catalog : BML-PW8810-0100 quantity : 100 µg clonality : monoclonal host : mouse conjugate : nonconjugated clone name : FK2 reactivity : southern flounder, Saccharomycetales, human, mouse, rat, fruit fly application : curing bronchitis at home

The dynamics of histone H2A ubiquitination in HeLa cells

Multiubiquitin Chain Monoclonal Antibody (Clone FK2)

WebWestern blots were developed using PVDF membranes and A-106 anti-ubiquitin primary antibody at 0.5 µg/ml followed by HRP-labeled anti-mouse (R&D Systems # HAF007) … WebCreative Biolabs offers high-quality Mouse Anti-Ubiquitin Monoclonal Antibody (FK2), Conjugated to boost neuroscience research. Toggle navigation. Service . In Vitro Services. Cell Culture Models; ... New Antibodies to Phospho Alpha Synuclein and Tau; Pre-made AAV Center; New Fast Genetically Encoded Calcium Indicators: jGCaMP8; curing borderline personality disorderWebMouse Monoclonal Antibody Shop MilliporeSigma™ anti-Ubiquitin Clone: FK2, at Fishersci.com. Fisher Scientific ; ... All Primary Antibodies ST1200100UG Print MilliporeSigma™ anti-Ubiquitin Clone: FK2, ... curing brine for bacon

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Fk2 antibody

WebUbiquitin Antibody - Mouse Monoclonal antibody to Ubiquitin, FK2. Species Independent. Validated for use in Western Blot, IHC, ICC/IF, IP, and ELISA. WebMay 15, 2005 · Separation of ubiquitinated Syk using an antiubiquitin antibody. Washed platelets were activated with convulxin (100 ng/mL) for 15 seconds. Cells were lysed with RIPA buffer and treated with FK2 antibody for 1 hour at 4°C, followed by Protein A/G PLUS-agarose for an additional hour. Supernatants were treated with anti-Syk using the …

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WebClone FK2 Antibody Products. Clone FK2 is reported to be a monoclonal antibody specific to the human gene, UBB. Indicated applications for antibody clone FK2 include Western … WebSep 15, 2013 · The FK2 antibody is a mouse monoclonal recognizing both monoubiquitinated and polyubiquitinated conjugates. According to the manufacturer’s data sheet the FK2 antibody can recognize various types of ubiquitin chains; however, it is unknown if it has equal affinities for all seven ubiquitin chain linkages and for different …

WebMar 25, 2016 · We previously developed an FK2 antibody, which specifically recognizes poly-Ub moie … Polyubiquitination plays key roles in proteasome-dependent and independent cellular events, whereas monoubiquitination is involved in gene expression, DNA repair, protein-protein interaction, and protein trafficking. WebUbiquitin Antibody (FK2) [Unconjugated] Summary. This antibody will recover mono- and polyubiquitinated proteins in I.P. applications. This antibody detects mono- and …

WebAug 17, 2011 · The FK1 and FK2 monoclonal antibodies are used to differentiate between monoubiquitination and polyubiquitination, since FK2 detects both monoubiquitinated and polyubiquitinated proteins, while FK1 recognizes only polyubiquitinated proteins (Fujimuro et al., 1994). Therefore, to assess whether the ubiquitination of PSD-95 reflects ... WebThis Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin. Anti-Ubiquitin Mouse mAb (FK2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. MSDS. 分析证书.

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WebThe FK2 antibody works great in both human and rat samples (I haven't tried mouse) by western blot and immunofluorescence. This antibody should recognize the ubiquitin modifications (both mono... curing btWebRibosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is … curing blankets manufacturersWebSep 13, 2024 · The FK2 monoclonal ubiquitin antibody is a frequently used and widely-cited ubiquitin antibody that detects both mono- and polyubiquitin, but not free ubiquitin. … easy get to know you ice breakersWebThis Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the detection of Ubiquitin. Anti-Ubiquitin … curing black olives with saltWebThis Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the … curing beef with saltWebSep 10, 2016 · Scheme of the ELISA assay. (a) In the “forward” approach, microwell plates are coated with a polyclonal anti-EGFR intra, which captures the receptor from the lysate (1).Detection of Ub-EGFR or total EGFR (to normalize) is performed with primary monoclonal antibodies directed against Ub (FK2, 1) or EGFR extra (2), respectively, … easy get together foodWebClone FK2 is the most widely used ubiquitin antibody. This clone reacts with mono- and polyubiquitinated conjugates, but does not react with free ubiquitin. 1, 2 WARNING This product is not for human or veterinary use. Certificates of Analysis & Batch Specific Data Get Batch-Specific Data and Documents by Batch Number curing beef at home